Anti-Inflammatory Xanthones from the Twigs of Hypericum oblongifolium Wall.

作者：Ali Mumtaz^*; Arfan Muhammad; Ahmad Manzoor; Singh Kuldip; Anis Itrat; Ahmad Habib; Choudhary Muhammad Iqbal; Shah Muhammad Raza

来源：Planta Medica, 2011, 77(18): 2013-2018.

DOI：10.1055/s-0031-1280114

• Hypericum oblongifolium Wall.
• Clusiaceae
• xanthonolignoids
• hypericorin A
• hypericorin B
• anti-inflammatory (respiratory burst)

Two new xanthonolignoids, hypericorin A (1) and hypericorin B (2), along with five known new source compounds, a xanthonolignoid, kielcorin (3), 4-hydroxy-2,3-dimethoxyxanthone (4), 3,4,5-trihydroxyxanthone (5), 1,3-dihydroxy-5-methoxyxanthone (6) and 1,3,7-trihydroxyxanthone (7), were isolated from the stems (twigs) of Hypericum oblongifolium Wall. The structures of the new compounds were deduced on the basis of spectroscopic techniques (EI-MS, HREI-MS, (1)H NMR, (13)C NMR, HMQC, HMBC, and NOESY). We also report herein for the first time the single crystal X-ray structure of compound 6. Compounds 1-7 were screened for their in vitro anti-inflammatory (respiratory burst) inhibiting activities using isolated human neutrophils; compounds 1, 2, 3, 5, and 7 showed significant activities (IC(50) = 816.23 +/- 73.30, 985.20 +/- 55.80, 965.21 +/- 65.80, 907.20 +/- 50.80, 975.20 +/- 81.10 mu M, respectively), compound 6 showed moderate activity (IC(50) = 2500.85 +/- 50.50 mu M), while compound 4 was totally inactive at 1000 mu g/mL as compared to the positive control used, indomethacin (IC(50) = 757.99 +/- 5.90 mu M), and aspirin (IC(50) = 279.44 +/- 4.40 mu M). Compound 4 was also inactive in comparison with other tested Hypericum compounds.

• 出版日期2011-12