Diverse Role of Conformational Dynamics in Carboxypeptidase A-Driven Peptide and Ester Hydrolyses: Disclosing the "Perfect Induced Fit" and "Protein Local Unfolding'' Pathways by Altering Protein Stability

作者：Shushanyan Mikhael; Khoshtariya Dimitri E^*; Tretyakova Tatyana; Makharadze Maya; van Eldik Rudi

来源：Biopolymers, 2011, 95(12): 852-870.

DOI：10.1002/bip.21688

• carboxypeptidase A
• catalytic mechanisms
• protein stabilization/destabilization
• substrate activation/inhibition
• activation parameters
• conformational dynamics

Catalytic mechanisms of carboxypeptidase A (CPA) are well known for their diversity and the relative inaccessibility for a decisive comprehension. Recent encouraging attempts through modern computational techniques promoted new challenges for the complementary experimental endeavors. In this work, we have applied the stopped-flow technique and the method of reaction progress curve fitting to extract kinetic parameters for the CPA-catalyzed hydrolyses of smaller (typical) peptide and ester substrates, known for their strong activating/inhibiting impact, thus to which the traditional method of "initial rates'' is not applicable. Our approach that innately implies the overall constancy of the affecter (substrate plus "active'' product) concentration, made it possible to rigorously determine the physically meaningful "effective'' values for the catalytic and Michaelis constants under diverse experimental conditions including variable temperature and urea or trimethylamine N-oxide concentrations. Analysis of the obtained results allowed for: (i) the further substantiation of diverse mechanistic patterns for archetypal specific peptide and ester substrates, (ii) testing and disclosure of intrinsic links between the stabilizing/destabilizing and activating/inhibiting effects for the important model enzyme, CPA, and (iii) tentative explanation of a distinct activating/inhibiting impact of these substrates through the strong specific interaction of their benzyl (Bz) moiety with the substrate binding S-3 subsite of CPA. We have demonstrated that stabilization of CPA either through the interaction with an extra Bz moiety (belonging to another substrate or to the product) leads to the increase of its catalytic power with respect to the specific peptide substrate and to its decrease with respect to the counterpart ester substrate. We conjecture that the catalytic mechanisms operating in these two cases include: (a) the "promoted water'' mechanism for the peptide substrate that, seemingly, provides the almost "perfect induced fit'' (low-barrier conformational adaptation), and (b) presumably, the "anhydride intermediate'' mechanism for the ester substrate that, anyway, requires substantial conformational rearrangement (in fact, "partial or local unfolding'') of the protein environment in the course of the rate-determining step.

• 出版日期2011-12