A method to investigate protein association with intact sealed mycobacterial membrane vesicles

作者：D'Lima Nadia G; Teschke Carolyn M^*

来源：Analytical Biochemistry, 2015, 485: 109-111.

DOI：10.1016/j.ab.2015.06.023

• Bacterial protein secretion
• Membrane association
• Mycobacteria

In mycobacteria, probing the association of cytoplasmic proteins with the membrane itself, as well as with integral or peripheral membrane proteins, is limited by the difficulty in extracting intact sealed membrane vesicles due to the complex cell wall structure. Here we tested the association of Mycobacterium tuberculosis SecA1 and SecA2 proteins with intact membrane vesicles by a flotation assay using iodixanol density gradients. These protocols have wide applications for studying the association of other mycobacterial cytoplasmic proteins with the membrane and membrane-associated proteins.

• 出版日期2015-9-15