A structural phylogeny for understanding 2-oxoacid oxidoreductase function

作者：Gibson Marcus I; Chen Percival Yang Ting; Drennan Catherine L^*

来源：Current Opinion in Structural Biology, 2016, 41: 54-61.

DOI：10.1016/j.sbi.2016.05.011

2-Oxoacid:ferredoxin oxidoreductases (OFORs) are essential enzymes in microbial one-carbon metabolism. They use thiamine pyrophosphate to reversibly cleave carbon carbon bonds, generating low potential (similar to-500 mV) electrons. Crystallographic analysis of a recently discovered OFOR, an oxalate oxidoreductase (OOR), has provided a second view of OFOR architecture and active site composition. Using these recent structural data along with the previously determined structures of pyruvate:ferredoxin oxidoreductase, structure function relationships in this superfamily have been expanded and re-evaluated. Additionally, structural motifs have been defined that better serve to distinguish one OFOR subfamily from another and potentially uncover novel OFORs.

• 出版日期2016-12
• 单位MIT