An intracellular glucose biosensor based on nanoflake ZnO

作者：Fulati Alimujiang^*; Ali Syed M Usman; Asif Muhammad H; Alvi Naveed Ul Hassan; Willander Magnus; Brannmark Cecilia; Stralfors Peter; Borjesson Sara I; Elinder Fredrik; Danielsson Bengt

来源：Sensors and Actuators B: Chemical , 2010, 150(2): 673-680.

DOI：10.1016/j.snb.2010.08.021

• Glucose oxidase (GOD)
• Intracellular
• Potentiometric biosensor
• Nanoflake ZnO
• Nafion membrane

In this study a potentiometric intracellular glucose biosensor was fabricated by immobilization of glucose oxidase on nanoflake ZnO Nanoflake ZnO with a wall thickness around 200 nm was grown on the tip of a borosilicate glass capillary and used as a selective intracellular glucose biosensor for the measurement of glucose concentrations in human adipocytes and frog oocytes The results showed a fast response within 4s and a logarithmic linear glucose-dependent electrochemical potential difference over a wide range of glucose concentration (500 nM-10 mM) Our measurements of intracellular glucose were consistent with the values of intracellular glucose concentrations reported in the literature The monitoring capability of the sensor was demonstrated by following the increase in the intracellular glucose concentration induced by insulin in adipocytes and frog oocytes In addition the nanoflake ZnO material provided 1 8 times higher sensitivity than previously used ZnO nanorods under the same conditions Moreover the fabrication method in our experiment is simple and the resulting nanosensor showed good performance in sensitivity stability selectivity reproducibility and anti-interference All these results de

• 出版日期2010-10-28