Differentiation Between Hepatocellular Carcinoma and Colorectal Cancer Liver Metastases on High-Resolution Magic Angle Spinning Spectroscopy: Preliminary Study

作者：Kim So Yeon; Kim Siwon; Woo Chul Woong; Byun Jae Ho; Lee Seung Soo; Lee Moon Gyu; Kim Haeryoung; Lee Kyoung Ho; Kim Young Hoon; Cho Jai Young; Kim Suhkmann; Lee Jin Seong^*

来源：Applied Magnetic Resonance, 2014, 45(1): 19-35.

DOI：10.1007/s00723-013-0501-7

To explore the potential of high-resolution magic angle spinning (HRMAS) H-1 nuclear magnetic resonance (NMR) spectroscopy for differentiation and metabolite characterization of hepatocellular carcinoma (HCC) and colorectal liver metastases (CRLM), we prospectively included 21 pathologically confirmed malignant hepatic tumors (8 HCC and 13 CRLM) and 26 non-tumorous hepatic parenchyma from 26 patients who underwent hepatic tumor resection. Using intact tissue samples obtained during surgery, HRMAS H-1 NMR spectroscopy was performed at 11.7 T. All observable metabolite signals were acquired using a water-presaturated standard one-dimensional Carr-Purcell-Meiboom-Gill sequence. Metabolomic profiles contributing to the differentiation of HCC and CRLM and of each tumor and non-tumorous hepatic parenchyma were represented by orthogonal partial least squares discriminant analysis (OPLS-DA) and loading plots. Metabolite intensity normalized by total spectral intensities in both tumors was compared using student%26apos;s t tests. OPLS-DA and loading plots demonstrated good separation between tumors and non-tumorous hepatic parenchyma. The metabolomic characteristics of HCC showed separation from those of CRLMs according to OPLS-DA. Compared with CRLM, HCC showed significantly elevated levels of glucose (P %26lt; 0.01) and sn-Glycero-3-phosphocholine (P %26lt; 0.01), and decreased levels of hypoxanthine (P = 0.04). HCC and CRLM could be differentiated by the metabolic profile using HRMAS H-1 NMR spectroscopy.

• 出版日期2014-1