Development of Gateway Binary Vectors R4L1pGWB Possessing the Bialaphos Resistance Gene (bar) and the Tunicamycin Resistance Gene as Markers for Promoter Analysis in Plants

作者：Tanaka Yuji; Shibahara Kenta; Nakagawa Tsuyoshi^*

来源：Bioscience Biotechnology and Biochemistry, 2013, 77(8): 1795-1797.

DOI：10.1271/bbb.130405

• binary vector
• Gateway cloning
• plant transformation
• promoter
• reporter

We have developed a new series of R4L1 Gateway binary vectors (R4L1pGWB), which carry the bialaphos resistance gene (bar) or the UDP-N-acetylglucosamine: dolichol phosphate N-acetylglucosamine-1-P transferase (GPT) gene as selection markers that confer BASTA and tunicamycin resistance on plants respectively. R4L1pGWBs have an attR4-attL1-reporter and can accept an attL4-promoter-attR1 entry clone for easy construction of an attB4-promoter-attB1-reporter clone. The new R4L1pGWBs facilitate promoter:reporter analysis in pre-existing transgenic plants that are resistant to kanamycin or hygromycin.

• 出版日期2013-8