Mechanisms of peroxynitrite-induced [Ca2(i)(+]) increase in single neuronal cell

作者：Yi Y; Cai D; Gao ZH; Yang XL; Qu AL; Xu HB^*; Wang XM; Han SP

来源：Chinese Science Bulletin, 1998, 43(20): 1727-1732.

DOI：10.1007/BF02883974

• peroxynitrite (ONOO-)
• intracellular calcium concentration ([Ca2+](i))
• MN9D cells

The mechanism of peroxynitrite (ONOO-)-induced [Ca2+ ](i) increase in single MN9D cell (Dopaminergic neuroblastoma cell line) was studied by using Fura-2 microfluorometric technique. The results show that ONOO- caused a rapid increase of [Ca2+](i) when ONOO- was puffed to the cell. Removing Ca2+ from the bath or using calcium channel antagonist (CdCl2, Nifedipine) greatly inhibited the [Ca2+](i) increase induced by ONOO-, suggesting that the opening of L-Ca2+ channel makes a great contribution to the [Ca2+](i) increase. The effect of sulfhydryl reductive agent (DTT) on ONOO- -induced [Ca2+](i) increase suggests that ONOO- -activating L-Ca2+ channel is partly related to its oxidative speciality.

• 出版日期1998-10
• 单位华中科技大学