科研之友
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摘要
An amperometric biosensor with pea seedling amine oxidase and screen printed carbon electrode modified with MnO2 for determining biogenic amines (BAs) was designed. The bio-component was immobilized with Nafion solution. The enzymatically produced hydrogen peroxide was determined to quantify BAs. All measurements were performed in flow injection analysis system with phosphate buffer (66 mM, pH 7.5) as mobile phase at +400 mV?vs. Ag/AgCl. A linear correlation was found for cadaverine and putrescine from 150 mu M, for tyramine and histamine from 10300 mu M respectively. The limits of detection were determined to be 0.3 mu M for cadaverine and putrescine and 3.0 mu M for tyramine and histamine. The sensor was used to quantify BAs in chicken meat samples.
- 出版日期2013-1
