An Assessment of the Utility of Universal and Specific Genetic Markers for Opium Poppy Identification

作者：Lee Eun J^*; Hwang In K; Kim Nam Y; Lee Kyung L; Han Myun S; Lee Yang H; Kim Mu Y; Yang Moon S

来源：Journal of Forensic Sciences, 2010, 55(5): 1202-1208.

DOI：10.1111/j.1556-4029.2010.01423.x

• forensic science
• Papaver somniferum L
• genetic markers
• morphine biosynthesis
• profiling
• narcotic control

The proper identification of illicit plants such as Papaver somniferum L (opium poppy) is important for law enforcement agencies. The identification of opium poppy was presently tested using 10 genetic markers that are universal for all plants or specific to a few poppy plants. The genetic distances of universal markers such as nuclear internal transcribed spacer (ITS), 18S rRNA, plastid rbcL, and trnL-trnF intergenic spacer (IGS) of 14 species included in the Papaveraceae and Fumariaceae family were acquired by sequence comparisons. Both the ITS region and trnL-trnF IGS showed high levels of interspecific divergence. Six Papaver genera-specific markers were developed from coding regions involved in morphine biosynthesis. Three markers (TYDC, NCS, and BBE) produced amplicons only in opium poppy, providing a presence/absence test for opium poppy, while three additional markers (CYP80B1, SAT, and COR) were genus specific. These 10 markers might be useful for the forensic DNA analysis of opium poppy.

• 出版日期2010-9