Association Between Single Nucleotide Polymorphisms of the Interleukin-4 Gene and Atopic Dermatitis

作者：Gharagozlou Mohammad; Behniafard Nasrin; Amirzargar Ali Akbar; Hosseinverdi Sima; Sotoudeh Soheila; Farhadi Elham; Khaledi Mojdeh; Aryan Zahra; Moghaddam Zahra Gholizadeh; Mahmoudi Maryam; Aghamohammadi Asghar; Rezaei Nima^*

来源：Acta Dermatovenerologica Croatica, 2015, 23(2): 96-100.

• atopic dermatitis
• polymorphism
• single nucleotide
• interleukin-4 gene

Atopic dermatitis (AD) is an inflammatory skin disease in which both genetic and environmental factors seem to be involved. Several studies investigated the association of certain genetic factors with AD in different ethnic groups, but conflicting data were obtained. This study was performed to check the possible association between single nucleotide polymorphisms (SNPs) of interleukin 4 (IL-4) and the IL-4 receptor a chain (IL-4R alpha) and AD in a group of Iranian patients. The allele and genotype frequencies of genes encoding for IL-4 and IL-4Ra were investigated in 89 patients with AD in comparison with 139 healthy controls, using methods based on polymerase chain reaction sequence-specific primers. The most frequent alleles of IL-4 in patients were T at -1098 (P<0.001, odds ratio (OR)=2.35), C at -590 (P<0.001, OR=4.84) and C at -33 (P=0.002, OR=2.08). The most frequent genotypes of IL-4 in patients were TT, CC, and CC at positions -1098 (P<0.001, OR=3.59), -590 (P<0.001, OR=31.25) and -33 (P<0.001, OR=3.46), respectively. We found a significant lower frequency of GT at -1098 GT, TC at -590, and TC at -33 in patients. There were no statistically significant differences in the frequency of alleles and genotypes of IL-4Ra gene at position +1902. A strong positive association was seen between TCC haplotype and AD (68% in patients vs. 23.4% in controls, P<0.001, OR=8.91). We detected a significantly lower frequency of TTC, GCC, and TTT haplotypes (P<0.001, OR=0.02, P<0.001, OR=0.40, P<0.001, OR=0.39, respectively) in patients compared to controls. A significant association between the polymorphisms of the IL-4 gene promoter at positions -1098,-590, and -33 and AD was detected in the Iranian population.

• 出版日期2015