Autologous engineering of cartilage

作者：Emans Pieter J^*; van Rhijn Lodewijk W; Welting Tim J M; Cremers Andy; Wijnands Nina; Spaapen Frank; Voncken J Willem; Shastri V Prasad

来源：Proceedings of the National Academy of Sciences, 2010, 107(8): 3418-3423.

DOI：10.1073/pnas.0907774107

• agarose
• hypoxia
• in vivo bioreactor
• periosteum
• regenerative therapies

Treatment of full-thickness damage to hyaline cartilage is hampered by the limited availability of autologous healthy cartilage and the lengthy, cost-prohibitive cell isolation and expansion steps associated with autologous cartilage implantation (ACI). Here we report a strategy for de novo engineering of ectopic autologous cartilage (EAC) within the subperiosteal space (in vivo bioreactor), through the mere introduction of a biocompatible gel that might promote hypoxia-mediated chondrogenesis, thereby effectively overcoming the aforementioned limitations. The EAC is obtained within 3 wk post injection of the gel, and can be press-fit into an osteochondral defect where it undergoes remodeling with good lateral and subchondral integration. The implanted EAC showed no calcification even after 9 mo and attained an average O'Driscoll score of 11 (versus 4 for controls). An "on demand" autologous source of autologous cartilage with remodeling capacity is expected to significantly impact the clinical options in repair of trauma to articular cartilage.

• 出版日期2010-2-23