Development of a new procedure for the determination of captopril in pharmaceutical formulations employing chemiluminescence and a multicommuted flow analysis approach

作者：Lima Manoel J A; Fernandes Ridvan N; Tanaka Auro A; Reis Boaventura F^*

来源：Luminescence, 2016, 31(1): 288-294.

DOI：10.1002/bio.2960

• captopril
• chemiluminescence
• multicommuted flow analysis
• solenoid mini-pumps
• green chemistry

This paper describes a new technique for the determination of captopril in pharmaceutical formulations, implemented by employing multicommuted flow analysis. The analytical procedure was based on the reaction between hypochlorite and captopril. The remaining hypochlorite oxidized luminol that generated electromagnetic radiation detected using a home-made luminometer. To the best of our knowledge, this is the first time that this reaction has been exploited for the determination of captopril in pharmaceutical products, offering a clean analytical procedure with minimal reagent usage. The effectiveness of the proposed procedure was confirmed by analyzing a set of pharmaceutical formulations. Application of the paired t-test showed that there was no significant difference between the data sets at a 95% confidence level. The useful features of the new analytical procedure included a linear response for captopril concentrations in the range 20.0-150.0 mu mol/L (r=0.997), a limit of detection (3 sigma) of 2.0 mu mol/L, a sample throughput of 164 determinations per hour, reagent consumption of 9 mu g luminol and 42 mu g hypochlorite per determination and generation of 0.63 mL of waste. A relative standard deviation of 1% (n=6) for a standard solution containing 80 mu mol/L captopril was also obtained.

• 出版日期2016-2