A novel study of antibacterial activity of copper iodide nanoparticle mediated by DNA and membrane damage

作者：Pramanik Arindam; Laha Dipranjan; Bhattacharya Debalina; Pramanik Panchanan; Karmakar Parimal^*

来源：Colloids and Surfaces B: Biointerfaces , 2012, 96: 50-55.

DOI：10.1016/j.colsurfb.2012.03.021

• Bactericidal
• Copper Iodide Nanoparticle
• Minimum bactericidal concentration
• Minimum Inhibitory concentration
• Reactive oxygen species

In this article potential activity of nanoparticles (NPs) of copper iodide (Cul) as an antibacterial agent has been presented. The nano particles are synthesized by co-precipitation method with an average size of 8 nm as determined by Transmission Electron Microscope (TEM). The average charge of the NPs is -21.5 mV at pH 7 as obtained by zeta potential measurement and purity is determined by XRD. These NPs are able to kill both gram positive and gram negative bacteria. Among the bacteria tested, DH5 alpha is more sensitive but Bacillus subtilis is more resistant to NPs of Cul. Consequently, the MIC and MBC values of DH5 alpha is least (0.066 mg/ml and 0.083 mg/ml respectively) and B. subtilis is highest (0.15 mg/ml and 0.18 mg/ml respectively) among the tested bacterial strains. From our studies it is inferred that Cul NPs produce reactive oxygen species (ROS) in both gram negative and gram positive bacteria and it also causes ROS mediated DNA damage for the suppression of transcription as revealed by reporter gene assay. Probably ROS is formed on the surface of NPs of Cul in presence of amine functional groups of various biological molecules. Furthermore they induce membrane damage as determined by atomic force microscopy (AFM). Thus production of ROS and membrane damage are major mechanisms of the bactericidal activity of these NPs of Cul.

• 出版日期2012-8-1