Development of microsatellites for Southern Darwin%26apos;s frog Rhinoderma darwinii (Dumeril %26 Bibron, 1841)

作者：Fuentes Navarrete Marina; Gomez Uchida Daniel; Gallardo Escarate Cristian; C****es Aguirre Cristian B; Galleguillos Ricardo; Carlos Ortiz Juan

来源：Conservation Genetics Resources, 2014, 6(4): 1035-1037.

DOI：10.1007/s12686-014-0279-4

• Southern Darwin%26apos
• s frog
• Rhinoderma darwinii
• Microsatellites
• Next generation sequencing

The Southern Darwin%26apos;s frog Rhinoderma darwinii is a charismatic, mouth-brooding amphibian endemic to temperate forests of South America with a Vulnerable conservation status according to the IUCN Red List. We developed microsatellite markers from next generation sequence data that will aid genetic monitoring during and after re-introduction efforts. Using bioinformatics we characterized 3,521 perfect microsatellite repeats and designed primers for 35 of them. From these, 23 were polymorphic and amplified reliably. Number of alleles varied between 2 and 15, allele sizes varied between 84 and 299 bp, and observed heterozygosities varied between 0.105 and 0.904. These microsatellites represent a valuable resource to aid recovery of threatened Southern Darwin%26apos;s frog populations.

• 出版日期2014-12