科研之友
微信
新浪微博
Facebook
分享链接
摘要
Multienzyme complexes (MECs) in nature exhibit highly efficient catalytic mechanisms in reaction cascades. Different strategies have been developed to colocalize enzymes on nanocarriers to improve multienzyme catalytic efficiency by mimicking MEC structure and function. Numerous studies have indicated that the spatial arrangement and orientation of multiple enzymes in confined spaces are critical in facilitating cooperative enzymatic activity in multienzyme colocalization. Biomolecule scaffolds based on DNA hybridization have attracted great attention because of their unique effective control of the relative positions of different enzymes in multienzyme colocalization. To demonstrate this concept, glucose oxidase (GOX) and horseradish peroxidase (HRP) were colocalized onto polystyrene nanopartides via specific DNA hybridization. Colocalization of GOX and HRP was evidenced by Forster resonance energy transfer studies of the dyes labeling the two tag DNAs. Finally, it was observed that colocalization of GOX and HRP via DNA hybridization significantly improved both the overall reaction efficiency and the storage shelf life compared with those of the single enzyme immobilization mixture control. In summary, DNA-directed colocalization of multiple enzymes on nanoparticles is an effective way to control the relative positioning of enzymes to mimic MECs and enhance catalytic activity.
- 出版日期2015-10-28
