Adsorption of Milk Proteins (beta Casein and beta-Lactoglobulin) and BSA onto Hydrophobic Surfaces

作者：Perez Fuentes Leonor; Drummond Carlos; Faraudo Jordi^*; Bastos Gonzalez Delfi

来源：Materials, 2017, 10(8): 893.

DOI：10.3390/ma10080893

• proteins
• QCM
• MD simulations
• hydrophobic effect
• ion condensation
• electrokinetic mobility

Here, we study films of proteins over planar surfaces and protein-coated microspheres obtained from the adsorption of three different proteins (beta-casein, beta-lactoglobulin and bovine serum albumin (BSA)). The investigation of protein films in planar surfaces is performed by combining quartz crystal microbalance (QCM) and atomic force microscopy (AFM) measurements with all-atomic molecular dynamics (MD) simulations. We found that BSA and beta-lactoglobulin form compact monolayers, almost without interstices between the proteins. However, beta-casein adsorbs forming multilayers. The study of the electrokinetic mobility of protein-coated latex microspheres shows substantial condensation of ions from the buffer over the complexes, as predicted from ion condensation theories. The electrokinetic behavior of the latex-protein complexes is dominated by the charge of the proteins and the phenomenon of ion condensation, whereas the charge of the latex colloids plays only a minor role.

• 出版日期2017-8