Addressing, amplifying and switching DNAzyme functions by electrochemically-triggered release of metal ions

作者：Freage Lina; Trifonov Alexander; Tel Vered Ran; Golub Eyal; Wang Fuan; McCaskill John S; Willner Itamar^*

来源：Chemical Science, 2015, 6(6): 3544-3549.

DOI：10.1039/c5sc00744e

The design of artificial cells, which mimic the functions of native cells, is an ongoing scientific goal. The development of stimuli-responsive chemical systems that stimulate cascaded catalytic transformations, trigger chemical networks, and control vectorial branched transformations and dose-controlled processes, are the minimum requirements for mimicking cell functions. We have studied the electrochemical programmed release of ions from electrodes, which trigger selective DNAzyme-driven chemical reactions, cascaded reactions that self-assemble catalytic DNAzyme polymers, and the ON-OFF switching and dose-controlled operation of catalytic reactions. The addressable and potentialcontrolled release of Pb2+ or Ag+ ions into an electrolyte that includes a mixture of nucleic acids, results in the metal ion-guided selection of nucleic acids yielding the formation of specific DNAzymes, which stimulate orthogonal reactions or activate DNAzyme cascades.

• 出版日期2015