Detection of all Chlamydophila and Chlamydia spp. of veterinary interest using species-specific real-time PCR assays

作者：Pantchev Alexandra; Sting Reinhard; Bauerfeind Rolf; Tyczka Judith; Sachse Konrad^*

来源：Comparative Immunology, Microbiology and Infectious Diseases, 2010, 33(6): 473-484.

DOI：10.1016/j.cimid.2009.08.002

• Chlamydia
• Chlamydophila
• Mammals
• Zoonosis
• Domestic animals
• Diagnostics
• Real-time PCR

The aim of the present study was to analyse the occurrence of chlamydiae in several mammalian host species. Clinical samples that previously tested positive in a Chlarnydiaceae-specific real-time PCR were retested using six species-specific real-time PCR assays to identify the chlamydial species involved. Chlantydophila (Cp.) abortus was the agent most frequently found in cattle, sheep, horses, goats, and pigs. Detection in cattle of Cp. psittaci (11% of samples) and Chlatnydia (C.) suis (9%), as well as Cp. psittaci in a goat sample was somewhat unexpected. DNA of two different chlamydiae was identified in 56 (12.7%) of 440 samples tested. Cp. felis was the predominant species found in cats, while in guinea pigs and rabbits only Cp. caviae was detected. Interestingly, the latter two pathogens were also identified in samples from dogs. The data show that mixed chlamydial infections are not rare and suggest an extended host range of individual species.

• 出版日期2010-12