Application of the OECD 301F respirometric test for the biodegradability assessment of various potential endocrine disrupting chemicals

作者：Stasinakis Athanasios S^*; PetalaS Anastasios V; Mainais Daniel; Thomaidis Nikolaos S

来源：Bioresource Technology, 2008, 99(9): 3458-3467.

DOI：10.1016/j.biortech.2007.08.002

• biodegradation kinetics
• respirometry
• wastewater
• activated sludge
• EDCs

The biodegradability of several potential endocrine disrupting compounds, namely 4-n-nonylphenol (4-n-NP), nonylphenol monoethoxylate (NP1EO), nonylphenol diethoxylate (NP2EO), bisphenol A (BPA), triclosan (TCS), di-(2-ethylhexyl)-phthalate (DEHP), perfluorooctanoate (PFOA) and perfluorononanoate (PFNA) was evaluated in this study, using OECD method 301F (manometric respirometry test) and activated sludge as inoculum. According to the results, 4-n-NP and BPA meet the strict definition of ready biodegradability and they are not expected to be persistent during the activated sludge process. Partial biodegradation was observed for DEHP (58.7 +/- 5.7%, n = 3). TCS (52.1 +/- 8.5%, n = 3) and NP1EO (25.9 +/- 8.1%, n = 3), indicating their possible biodegradation in wastewater treatment systems, while no biodegradation was observed for NP2EO, PFOA and PFNA. Experiments in the co-presence of a readily biodegradable compound showed the absence of co-metabolic phenomena during 4-n-NP, BPA and TCS biodegradation. Using first order kinetics to describe biodegradation of the target compounds, half-lives of 4.3 +/- 0.6, 1.3 +/- 0.2, 1.8 +/- 0.5, 6.9 +/- 2.6 days were calculated for 4-n-NP, BPA, TCS and DEHP, respectively. Toxicity tests using marine bacterium Vibrio fischeri showed that biodegradation of 4-n-NP, NP1EO, BPA and TCS is a simultaneous detoxification process, while possible abiotic or biotic transformations of NP2EO, DEHP, PFOA and PFNA during respirometric test resulted to significant increase of their toxicities.

• 出版日期2008-6