A high throughput methodology for susceptibility testing of Mycobacterium tuberculosis isolates

作者：Rampersad Tashmin; Makume Mantha; Sobia Parveen; Sturm A Willem^*

来源：Journal of Microbiological Methods, 2018, 146(3): 64-67.

DOI：10.1016/j.mimet.2018.02.001

• Mycobacterium tuberculosis
• Drug susceptibility test
• MIC
• Agar-dilution
• Multi-point inoculation

MICs for eleven anti-TB drugs with M. tuberculosis isolates were obtained by means of agar dilution with multi point inoculation. The results were compared with classic agar dilution and the MTT assay. The multi-point inoculation method was reproducible with all drugs and correlated with classic agar dilution and MTT assay. This methodology can be used for routine breakpoint drug susceptibility testing (DST) and for MIC determination.

• 出版日期2018-3