A Novel, Universal and Sensitive Lateral-Flow Based Method for the Detection of Multiple Bacterial Contamination in Platelet Concentrations

作者：Wang, Jidong; Wang, Xiaohui; Li, Yuan; Yan, Shaoduo; Zhou, Qianqian; Gao, Bo; Peng, Jianchun; Du, Juan; Fu, Qiuxia; Jia, Shuaizheng; Zhang, Juankun^*; Zhan, Linsheng

来源：Analytical Sciences, 2012, 28(3): 237-241.

DOI：10.2116/analsci.28.237

• REAL-TIME PCR
• POLYMERASE
• ASSAY

In the present study, we aimed to develop a nucleic acid lateral-flow method for the rapid and sensitive detection of multiple bacteria that contaminate platelet concentrations (PCs). Polymerase chain reaction (PCR) amplicons were produced by a set of board-range primers that recognize the conserved region of bacteria 16S rDNA, followed by hybridization with both an FITC (fluorescein isothiocyanate)-labelled probe and biotin-labelled probe, and then a nucleic acid lateral-flow dipstick (LFD) assay. The LFD accurately identified 7 species of bacteria, but had no cross-reactivity with human genomic DNA. The limit of detection (LOD) of the LFD assay was as low as 10(1) copies/mu L of 16S rDNA for plasmid. In the case of spiked PCs without enrichment, the detection limit of LFD for K. pneumonia was 5 CFU/mL, 6.5 x 10(4) CFU/mL for the S. epidermidis and 35 CFU/mL for P. aeruginosa.

• 出版日期2012-3
• 单位天津科技大学