An enzyme-modulated oxygen-producing micro-system for regenerative therapeutics

作者：Abdi Syed Izhar Haider; Ng Sing Muk; Lim Jeong Ok^*

来源：International Journal of Pharmaceutics, 2011, 409(1-2): 203-205.

DOI：10.1016/j.ijpharm.2011.02.041

• Oxygen
• Controlled release
• Catalase
• Hypoxia
• In vitro assay

This study suggests the idea of treating oxygen as a drug in a biological environment and demonstrates that it will exhibit a dosage-dependent trend. To accomplish this, a micro-system was fabricated, having hydrogen peroxide as the oxygen-generating source, which was decomposed using catalase, a common enzyme found in nearly all living organisms. The relevance of the proposed micro-system was justified using cell viability assays under well-controlled and fixed conditions. This study was performed under two controlled conditions, normoxia and hypoxia, and tests were carried out using three different configurations of samples under each condition: direct addition of H(2)O(2), H(2)O(2) encapsulated with single layer, and H(2)O(2) encapsulated with double layers. This study demonstrates that the elegantly designed micro-system managed to control the decomposition of H(2)O(2) and avoided direct contact with cells, while also maintaining cell viability under a low oxygen environment.

• 出版日期2011-5-16