Modified sympathetic nerve regulation in AKAP5-null mice

作者：Han Chong; Tomita Hirofumi; Ohba Takayoshi; Nishizaki Kimitaka; Ogata Yoshiki; Matsuzaki Yasushi; Sawamura Daisuke; Yanagisawa Teruyuki; Osanai Tomohiro; Imaizumi Tadaatsu; Matsubara Atsushi; Adachi Takeshi; Ono Kyoichi; Okumura Ken; Murakami Manabu^*

来源：Biochemical and Biophysical Research Communications, 2016, 469(4): 897-902.

DOI：10.1016/j.bbrc.2015.12.057

• PKA
• Mouse
• Sympathetic nerve
• Parasympathetic nerve
• Heart rate

Genetic analyses have revealed an important association between A-kinase anchoring proteins (AKAPs) and the intracellular calcium modulating system. AKAP5, also known as AKAP79/150, is an anchoring protein between PKA and voltage-dependent calcium channels, ryanodine receptor-2, phospholamban and other molecules.
The aim of the present study was to elucidate the physiological importance of AKAP5 in the creation of cardiac rhythm using AKAP5-null mice.
ECG analysis showed a normal sinus rhythm and a decreased responsiveness to isoproterenol in AKAP5-null mice compared with wild-type mice. Analysis of heart rate variability revealed that the R-R interval was unstable in AKAP5- null mutants and that the low-frequency components had decreased, indicating that the tonus of the sympathetic nervous system was affected. Furthermore, the atrium of the AKAP5-null mice showed a decreased positive inotropic response to isoproterenol, indicating the involvement of AKAP5 in a PKA-dependent pathway. Thus, our present study revealed that AKAP5 plays a significant role in the regulation of sympathetic nerve activities.

• 出版日期2016-1-22