Anticancer activity studies of a ruthenium(II) polypyridyl complex against human hepatocellular (BEL-7402) cells

作者：Li, Wei; Han, Bing-Jie; Yao, Jun-Hua; Jiang, Guang-Bin; Lin, Gan-Jian; Xie, Yang-Yin; Huang, Hong-Liang^*; Liu, Yun-Jun

来源：Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy , 2015, 150: 127-134.

DOI：10.1016/j.saa.2015.05.032

• Ru(II) polypyridyl complex
• Scratch assay
• Cytotoxicity in vitro
• Reactive oxygen species
• Mitochondrial membrane potential
• Western blot analysis

A Ru(II) polypyridyl complex [Ru(bpy)(2)(HMSPIP)](ClO4)(2) (1) (bpy = 2,2'-bipyridine, HMSPIP =2(4-methylsulfonyl)phenyl-1H-imidazo[4,5-f][1,10] phenanthroline) was synthesized. The IC50 value of the complex against human hepatocellular cell BEL-7402 is 21.6 +/- 2.7 mu M. The complex shows no cytotoxic activity toward human lung adenocarcinoma cell A549, human osteosarcoma cell MG-63 and human breast cancer cell SK-BR-3 cells. It is easily for complex 1 to be taken up by BEL-7402 cells. The complex can enhance the reactive oxygen species (ROS) levels and induce the decrease in the mitochondrial membrane potential. The complex inhibits the cell growth in BEL-7402 cells at G2/M phase. Complex 1 can regulate the expression of Bcl-2 family proteins. The results show that the complex induces apoptosis of BEL-7402 cells through a ROS-mediated mitochondrial dysfunction pathway.

• 出版日期2015-11-5
• 单位广东药科大学; 中山大学