Arabidopsis Pumilio protein APUM5 suppresses Cucumber mosaic virus infection via direct binding of viral RNAs

作者：Huh Sung Un; Kim Min Jung; Paek Kyung Hee^*

来源：Proceedings of the National Academy of Sciences, 2013, 110(2): 779-784.

DOI：10.1073/pnas.1214287110

• plant defense
• Pumilio RNA binding protein
• TuMV

Posttranscriptional/translational regulation of gene expression is mediated by diverse RNA binding proteins and plays an important role in development and defense processes. Among the RNA-binding proteins, the mammalian Pumilio RNA-binding family (Puf) acts as posttranscriptional and translational repressors. An Arabidopsis Puf mutant, apum5-D, was isolated during a T-DNA insertional mutant screen for mutants with reduced susceptibility to Cucumber mosaic virus (CMV) infection. Interestingly, CMV RNA contained putative Pumilio-homology domain binding motifs in its 3%26apos; untranslated region (UTR) and internal places in its genome. APUM5 directly bound to the 3%26apos; UTR motifs and some internal binding motifs in CMV RNAs in vitro and in vivo. We showed that APUM5 acts as a translational repressor that regulates the 3%26apos; UTR of CMV and affects CMV replication. This study uncovered a unique defense system that Arabidopsis APUM5 specifically regulates CMV infection by the direct binding of CMV RNAs.

• 出版日期2013-1-8