Altered gene expression of NIDD in dorsal root ganglia and spinal cord of rats with neuropathic or inflammatory pain

作者：Chen Meng Ling; Cheng Chun; Lv Qing Shan; Guo Zhi Qin; Gao Ying; Gao Shang Feng; Li Xin; Niu Shu Qiong; Shi Shu Xian; Shen Ai Guo^*

来源：Journal of Molecular Histology, 2008, 39(2): 125-133.

DOI：10.1007/s10735-007-9144-z

• neuronal nitric oxide synthase
• nNOS-interacting DHHC domain-containing protein with dendritic mRNA
• pain
• complete Freund's adjuvant
• nerve injury

Nitric oxide and nitric oxide synthases are key players in synaptic plasticity events in spinal cord (SC), which underlies the chronic pain states. To date, little is known about the molecular mechanisms regulating the activity of nitric oxide synthases in nociceptive systems. The present study was aimed at the determination of the gene expression of nNOS-interacting DHHC domain-containing protein with dendritic mRNA (NIDD), a recently identified protein regulating nNOS enzyme activity, in rat SC and dorsal root ganglia (DRG) and studying its regulation in states of nociceptive hypersensitivity in a rat model of neuropathic or inflammatory pain. It was found that NIDD mRNA was predominantly expressed in nociceptive primary neurons and in neurons of the spinal dorsal horn (DH) and the number of NIDD-positive neurons in the corresponding DRG or SC increased significantly following induction of chronic hyperalgesia. Meanwhile, remarkable changes of nNOS were detected under such pain conditions. Our data suggest a potential role for NIDD in the maintenance of thermal pain hypersensitivity possibly via regulating the nNOS activity.

• 出版日期2008-4
• 单位复旦大学; 南通大学