An improved PCR-based amplification of unknown homologous DNA sequences

作者：Naghibalhossaini Fakhraddin^*; Moaddeb Afsaneh

来源：Biomolecular Engineering, 2006, 23(4): 209-212.

DOI：10.1016/j.bioeng.2006.04.002

• PCR
• cloning
• mismatched primer
• homologous DNA

The PCR primers used for cloning of evolutionary conserved genes or homologous DNA sequences are usually guessmer oligonucleotides. We introduce a simple way using Pfu polymerase to overcome possible PCR amplification failure because of 3'-end mismatches of guessed primers with the target DNA.

• 出版日期2006-9