A novel vaccine adjuvant comprised of a synthetic innate defence regulator peptide and CpG oligonucleotide links innate and adaptive immunity

作者：Kindrachuk Jason; Jenssen Havard; Elliott Melissa; Townsend Rebecca; Nijnik Anastasia; Lee Song F; Gerdts Volker; Babiuk Lorne A; Halperin Scott A; Hancock Robert E W^*

来源：Vaccine, 2009, 27(34): 4662-4671.

DOI：10.1016/j.vaccine.2009.05.094

• Adjuvant
• Innate defence regulator peptide
• Host defense peptide
• CpG DNA
• Oligodeoxynucleotides
• Toll-like receptors
• Formulation

There has been an increased demand for the development of novel vaccine adjuvants that lead to enhanced induction of protection from infectious challenges and development of immunological memory. A novel vaccine adjuvant was developed comprising a complex containing CpG oligonucleotide and the synthetic cationic innate defence regulator peptide HH2 that has enhanced immune modulating activities. The complex of HH2 and the CpG oligonucleotide 10101 was a potent inducer of cytokine/chemokine expression ex vivo, retained activity following extended storage, had low associated cytotoxicity, and upregulated surface marker expression in dendritic cells, a critical activity for a vaccine adjuvant. Immunization of mice with a coformulation of the HH2-CpG complex and pertussis toxoid significantly enhanced the induction of toxoid-specific antibody titres when compared to toxoid alone, inducing high titres of IgG1 and IgG2a, typical of a balanced Th1/Th2 response, and also led to high IgA titres. This study demonstrates the potential application of the HH2-CpG complex as a vaccine adjuvant.

• 出版日期2009-7-23