Development and validation of a high performance liquid chromatography method for determination of 6-benzyl-1-benzyloxymethyl-5-iodouracil (W-1), a novel non-nucleoside reverse transcriptase inhibitor and its application to a pharmacokinetic study in rats

作者：Lu, Ying-Yuan; Wang, Xin; Wang, Xiao-Wei; Liu, Jun-Yi; Li, Pu; Ren, Hong; Lou, Ya-Qing; Lu, Chuang; Zhang, Guo-Liang^*

来源：Biomedical Chromatography, 2015, 29(10): 1548-1552.

DOI：10.1002/bmc.3457

• 6-benzyl-1-benzyloxymethyl-5-iodouracil (W-1)
• HPLC
• pharmacokinetics
• rat

A sensitive and selective high-performance liquid chromatographic (HPLC) method for determination of 6-benzyl-1-benzyloxymethyl-5-iodouracil (W-1), a novel non-nucleoside reverse transcriptase inhibitor in rat plasma, was developed and validated. Chromatographic separation of W-1 and megestrol acetate (internal standard) was achieved on a reversed-phase C-18 column at 25 degrees C. The mobile phase was consisted of acetonitrile-water (60:40, v/v) and pumped at a flow rate of 1.0 mL/min. The ultraviolet (UV) detector was set at the absorption wavelength of 284 nm. The calibration curve for W-1 was linear over the concentration range of 0.01-8 mu g/mL and the lower limit of quantification was 10 ng/mL. The intra- and inter-day precision and accuracy were <8.9 and 5.3%, respectively. The extraction recoveries ranged from 97.9 to 101.6%. The validated HPLC method was successfully applied to a pharmacokinetic study of W-1 in rats.

• 出版日期2015-10
• 单位北京大学