Manual and automated reticulocyte counts

作者：Simionatto Mackelly; de Paula Josiane Padilha; Flores Chaves Michele Ana; Bortoloso Marcia; Cicchetti Domenic; Soares Leonart Maria Suely; do Nascimento Aguinaldo Jose^*

来源：Hematology, 2010, 15(6): 406-409.

DOI：10.1179/102453310X12647083621128

• Hematology
• reticulocytes count
• statistical analysis

Manual reticulocyte counts were examined under light microscopy, using the property whereby supravital stain precipitates residual ribosomal RNA versus the automated flow methods, with the suggestion that in the latter there is greater precision and an ability to determine both mature and immature reticulocyte fractions. Three hundred and forty-one venous blood samples of patients were analyzed of whom 224 newborn and the rest adults; 51 males and 66 females, with ages between 0 and 89 years, as part of the laboratory routine for hematological examinations at the Clinical Laboratory of the Hospital Universitario do Oeste do Parana. This work aimed to compare manual and automated methodologies for reticulocyte countings and evaluate random and systematic errors. The results obtained showed that the difference between the two methods was very small, with an estimated 0.4% systematic error and 3.9% random error. Thus, it has been confirmed that both methods, when well conducted, can reflect precisely the reticulocyte counts for adequate clinical use.

• 出版日期2010-12