A novel glucose sensor using lutetium phthalocyanine as redox mediator in reduced graphene oxide conducting polymer multifunctional hydrogel

作者：Al Sagur H; Komathi S; Khan M A; Gurek A G; Hassan A^*

来源：Biosensors and Bioelectronics, 2017, 92: 638-645.

DOI：10.1016/j.bios.2016.10.038

• Polyacrylic acid
• Reduced graphene oxide
• Polyaniline
• Lutetium phthalocyanine
• Glucose biosensor
• Multifunctional hydrogel

Herein, we report a scalable synthesis of multifunctional conducting polyacrylic acid (PAA) hydrogel (MFH) integrated with reduced grapheme oxide (rGO), vinyl substituted polyaniline (VS-PANI) and lutetium Phthalocyanine (LuPc2) as three dimensional robust matrix for glucose oxidase (GOx) immobilization (PAA-rGO/VS-PANI/LuPc2/GOx-MFH). We have integrated the multicomponents such as PAA with rGO, and VS-PANI through free radical polymerization using methylene bis-acrylamide, and ammonium persulphate as the cross linker and initiator. The LuPc2 was then doped to form multifunctional hydrogel (PAA-rGO/VS-PANI/LuPc2-MFH). Finally, biosensor was fabricated by immobilizing GOx into PAA-rGO/VS-PANI/LuPc2-MFH and subsequently used for electrochemical detection of glucose. The PAA-rGO/VS-PANI/LuPc2/GOx-MFH biosensor exhibited high sensitivity (15.31 mu A mM(-1) cm(-2)) for the detection of glucose over a concentration range of 2-12 mM with a low detection limit of 25 mu m. The PAA-rGO/VS-PANI/LuPc2-MFH biosensor showed a fast response time (1 s) to the addition of glucose with high storage stability of 3 months. The real sample analysis reveals that PAA-rGO/VS-PANI/LuPc2/GOx-MFH could be effectively used as an electrochemical biosensor in industrial as well clinical diagnosis.

• 出版日期2017-6-15