Multiple alkane hydroxylase systems in a marine alkane degrader, Alcanivorax dieselolei B-5

作者：Liu Chenli; Wang Wanpeng; Wu Yehui; Zhou Zhongwen; Lai Qiliang; Shao Zongze

来源：Environmental Microbiology, 2011, 13(5): 1168-1178.

DOI：10.1111/j.1462-2920.2010.02416.x

P>Alcanivorax dieselolei strain B-5 is a marine bacterium that can utilize a broad range of n-alkanes (C(5)-C(36)) as sole carbon source. However, the mechanisms responsible for this trait remain to be established. Here we report on the characterization of four alkane hydroxylases from A. dieselolei, including two homologues of AlkB (AlkB1 and AlkB2), a CYP153 homologue (P450), as well as an AlmA-like (AlmA) alkane hydroxylase. Heterologous expression of alkB1, alkB2, p450 and almA in Pseudomonas putida GPo12 (pGEc47 Delta B) or P. fluorescens KOB2 Delta 1 verified their functions in alkane oxidation. Quantitative real-time RT-PCR analysis showed that these genes could be induced by alkanes ranging from C(8) to C(36). Notably, the expression of the p450 and almA genes was only upregulated in the presence of medium-chain (C(8)-C(16)) or long-chain (C(22)-C(36)) n-alkanes, respectively; while alkB1 and alkB2 responded to both medium- and long-chain n-alkanes (C(12)-C(26)). Moreover, branched alkanes (pristane and phytane) significantly elevated alkB1 and almA expression levels. Our findings demonstrate that the multiple alkane hydroxylase systems ensure the utilization of substrates of a broad chain length range.

• 出版日期2011-5
• 单位厦门大学