A straightforward route to the synthesis of a surface-enhanced Raman scattering probe for targeting transferrin receptor-overexpressed cells

作者：Yang, Jing^*; Wang, Zhuyuan; Tan, Xuebin; Li, Jin; Song, Chunyuan; Zhang, Ruohu; Cui, Yiping

来源：Nanotechnology, 2010, 21(34): 345101.

DOI：10.1088/0957-4484/21/34/345101

• MEDIATED ENDOCYTOSIS
• GOLD NANOPARTICLES
• NANORODS
• SILVER
• SPECTROSCOPY
• TRAFFICKING
• NANOPROBES
• APOPTOSIS
• ACID
• TAGS

A tumor cell targeting surface-enhanced Raman scattering (SERS) probe has been successfully synthesized by using p-mercaptobenzoic acid (pMBA) as both the SERS reporter and the conjugation agent for attaching transferrin molecules, which shows experimentally the targeting ability for transferrin receptor-overexpressed HeLa cells and exhibits strong SERS signals when being incubated inside cells. To prove that the uptake of such a SERS probe is through a Tf-receptor-mediated endocytosis process, two control experiments: (1) HeLa cells being incubated with the probe at 4 degrees C and (2) HeLa cells being pre-blocked with free transferrin at 37 degrees C, were employed. The difference of SERS intensity between the transferrin-overexpressed HeLa cells and transferrin-pre-blocked HeLa cells indicates that the probe has the potential to selectively target tumor cells.

• 出版日期2010-8-27
• 单位东南大学