Improvement of growth and camptothecin yield by altering nitrogen source supply in cell suspension cultures of Camptotheca acuminata

作者：Pan, XW; Xu, HH; Liu, X; Gao, X; Lu, YT^*

来源：Biotechnology Letters, 2004, 26(22): 1745-1748.

DOI：10.1007/s10529-004-4580-2

• ammonium
• Camptotheca acuminata
• camptothecin
• nitrate
• two-stage culture

Nitrate at 70 mM gave the highest biomass of Camptotheca acuminata in suspension culture in MS medium, but a NH4+/NO3- molar ratio of 5:1 (giving a total of 40 mM N) gave the maximum camptothecin yield. A two-stage flask culture system was established to improve culture efficiency; cell dry weight, camptothecin content and yield was increased by 30%, 280% and 340%, respectively when compared with those of control, reaching up to 36 g l(-1), 0.36 mg g(-1), and 12.8 mg l(-1) , respectively.

• 出版日期2004-11
• 单位武汉大学