Anti-osteoporotic activity of sialoglycoproteins isolated from the eggs of Carassius auratus by promoting osteogenesis and increasing OPG/RANKL ratio

作者：Xia Guanghua; Wang Shanshan; He Min; Zhou Xiaochun; Zhao Yanlei; Wang Jingfeng^*; Xue Changhu

来源：Journal of Functional Foods, 2015, 15: 137-150.

DOI：10.1016/j.jff.2015.03.021

• Fish eggs
• Sialoglycoprotein
• OPG/RANKL ratio
• BMP-2
• BMD
• Osteogenesis

The anti-osteoporotic activities of a sialoglycoprotein isolated from the eggs of Carassius auratus (Ca-SGP). Ca-SGP was composed of 14.33% protein, 62.81% hexose, and 19.72% N-acetylneuraminic acid (Neu5Ac). In vitro experimental results showed that Ca-SGP significantly increased MC3T3-E1 pre-osteoblastic cells' proliferation activities, promoted cell differentiation and mineralization as evidenced by increasing the contents or activities of major markers, i.e. collagen type I (COL I), osteocalcin (OCN), alkaline phosphatase (ALP) and bone nodules. Ca-SGP significantly augmented protein expressions and secretions of bone morphogenetic protein 2 (BMP-2) and osteoprotegerin (OPG), meanwhile, decreased that of receptor activator of nuclear factor kappa B ligand (RANKL). In vivo experimental data further revealed that Ca-SGP significantly reduced bone turnover rates, increased serum OPG/RANKL ratio and enhanced tibia bone mineral density (BMD) in the model rats of ovariectomized (OVX)-induced osteoporosis. These results suggest that Ca-SGP could improve osteoporosis by promoting osteogenesis and inhibiting bone resorption via increasing OPG/RANKL ratio, which indicates that Ca-SGP is a potential candidate functional factor for osteoporosis prevention.

• 出版日期2015-5
• 单位中国海洋大学