NUCLEOTIDE-SEQUENCE OF A HIGHLY REPEATED DNA-SEQUENCE AND ITS CHROMOSOMAL LOCALIZATION IN ALLIUM-FISTULOSUM

作者：IRIFUNE K; HIRAI K; ZHENG J; TANAKA R; MORIKAWA H

来源：Theoretical and Applied Genetics, 1995, 90(3-4): 312-316.

• ALLIUM FISTULOSUM
• REPEATED DNA SEQUENCE
• IN SITU HYBRIDIZATION
• NUCLEOTIDE SEQUENCE
• C-BANDING

A highly repeated DNA sequence with a repeating unit of approximately 380 bp was found in EcoRV digests of the total genomic DNA of Allium fistulosum. Three independent clones containing this unit were isolated, and their repeating units sequenced. These units showed more than 94% sequence homology, and the copy number was estimated to be about 2.8 x 10(6) per haploid genome. In situ hybridization, with the repeating unit as a probe, and C-banding analyses indicated that the repeated DNA sequence of A. fistulosum is closely associated with the major C-heterochromatin in the terminal regions of all 16 chromosomes at mitotic metaphase. The characters of the repeating unit are similar to those of the A. cepa unit, which is taxonomically closely related to A. fistulosum.

• 出版日期1995-3