科研之友
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摘要
Environmental estrogens in the aquatic environment have been shown to be responsible for the feminization of fish. The estrogenic content of the Yangtze River (Nanjing section - referred to as the studied area herein) was assessed using a combination of bioassay and chemical analysis. The in vivo bioassay was conducted by exposing adult male goldfish (Carassius auratus) to different concentrations of river water (25%, 50% and 100%) sampled from three representative sections of the studied area. Chemical analysis of estrogens in water from the three representative sections was conducted using solid phase extraction-gas chromatograph (SPE-GC) detection. The assay showed significant serum vitellogenin (VTG) and 17 beta-estradiol (E(2)) induction and gonad atrophy in the treated fish. The strength of in vivo estrogenic responses in the three representative sections is in the order of Jiangxinzhou section > Daqiao section > Sanchahe section. The result is consistent with the levels of water estrogens determined from the chemical analysis. Steroidal estrogens were the major causal agents responsible for the estrogenic responses in the Jiangxinzhou and Daqiao sections, while phenolic estrogens were the main contributors in the Sanchahe section. The results of these in vivo bioassay and chemical analysis demonstrate that fish in the Yangtze River are exposed to environmental estrogens and are at a risk of feminization.
