A Novel Fluorescent Biosensor for Detection of Silver Ions Based on Upconversion Nanoparticles

作者：Long, Qian; Wen, Yanqing; Li, Haitao; Zhang, Youyu^*; Yao, Shouzhuo

来源：Journal of Fluorescence, 2017, 27(1): 205-211.

DOI：10.1007/s10895-016-1947-4

• Upconversion nanoparticles
• Ag+
• O-phenylenediamine
• Inner filter effects

In this paper, we report a simple and sensitive fluorescent biosensor for the quantitative analysis of silver ions (Ag+) by using NaYF4:Yb3+, Tm3+ upconversion nanoparticles (UCNPs). Ag+ could oxidize o-phenylenediamine (OPD) to the oxidized OPD (oxOPD) directly. The fluorescence of UCNPs can be significantly quenched by oxOPD through inner filter effects (IFE). Under the optimized conditions, the Ag+ concentration is proportional to the changes of the fluorescence intensity of UCNPs. The proposed method shows high selectivity and Ag+ could be quantitatively detected in the range of 0 to 0.5 mM with a low detection limit of 33 nM for Ag+. The selectivity and sensitivity of the detection can also be satisfactory. More importantly, this method has potential in practical application to detect Ag+ in real samples without interference.

• 出版日期2017-1
• 单位湖南师范大学