DYNAMIC QTL ANALYSIS OF CHLOROPHYLL CONTENT DURING GRAIN FILLING STAGE IN WINTER WHEAT (TRITICUM AESTIVUM L.)

作者：Yang, Bin; Yan, Xue; Wang, Huiyan; Li, Xiaoyu; Ma, Haoxiang; Wang, Shuguang; Sun, Daizhen^*; Jing, Ruilian

来源：Romanian Agricultural Research, 2016, 33: 77-85.

• chlorophyll content
• marker-assisted selection
• quantitative trait loci
• grain filling stage
• conditional and unconditional mapping
• wheat

A recombinant inbred line (RIL) population consisting of 306 lines was constructed from a cross between two winter wheat (Triticum aestivum L.) cultivars, Hanxuan 10 and Lumai 14. The dynamic quantitative trait loci (QTLs) for chlorophyll content of flag leaves were detected using the RIL population by a combination of unconditional and conditional mapping method. Chlorophyll content was measured with a SPAD meter at seven stages of grain filling under two water conditions (well-watered and drought-stress). Thirty-four simple sequence repeat markers used in this study were derived from the candidate QTLs for chlorophyll content previously detected in a doubled haploid population of the same cross as the RIL. In the entire grain-filling period, five additive QTLs and one pair of epistatic QTLs were detected by unconditional mapping, while seven additive QTLs and one pair of epistatic QTLs were detected by conditional mapping. These QTLs for chlorophyll content were distributed on six chromosomes: 3B, 3D, 4B, 5A, 6A and 7A. The phenotypic variation explained by individual QTLs ranged from 2.12% to 12.80%. A major QTL (QChl-5A.1) was detected at multiple stages during grain filling, unaffected by genetic background and environmental conditions and might have great prospects for use in marker-assisted selection (MAS) breeding. The combination of unconditional and conditional QTL approach allowed for better understanding of both dynamic and static genetic information on the expression of QTLs for chlorophyll content of flag leaves during grain filling of wheat, providing reference data for marker-assisted selection breeding of high-yielding wheat cultivars.

• 出版日期2016
• 单位中国农业科学院; 山西省农业科学院谷子研究所; 山西农业大学