科研之友
微信
新浪微博
Facebook
分享链接
摘要
A method for the separation and purification of indole-3-acetic acid and abscisic acid based on double-direction dispersive liquid-liquid microextraction coupled with high-performance liquid chromatography was developed. The reverse-direction dispersive liquid-liquid microextraction procedure was very efficient in the purification of the hormones. The forward-direction dispersive liquid-liquid microextraction procedure was very efficient in the enrichment of the analytes. Under optimum conditions, a linear response was observed over the ranges of 0.2 mu g/g to 200.0 mu g/g for indole-3-acetic acid and 0.1 mu g/g to 200.0 mu g/g for abscisic acid, with correlation coefficients of 0.9967 and 0.9983. The limits of detection for indole-3-acetic acid and abscisic acid were 0.02 mu g/g and 0.01 mu g/g. The recoveries by standard addition were 91.4% and 88.6% for indole-3-acetic acid and abscisic acid with corresponding relative standard deviations of 4.5% and 6.1% (n=5). The developed method was used for the preconcentration and determination of indole-3-acetic acid and abscisic acid in Arabidopsis thaliana, and satisfactory results were obtained.
- 出版日期2015-3-4
- 单位河北农业大学
