Determination of the triterpene glycosides in sea cucumbers by liquid chromatography with evaporative light scattering and mass spectrometry detection

作者：Yang Jie; Wang Yuanhong; Zhang Ran; Jiang Tifngfu; Lv Zhihua^*

来源：Journal of Separation Science, 2015, 38(7): 1117-1122.

DOI：10.1002/jssc.201401253

• Electrospray ionization mass spectrometry
• Evaporative light scattering detection
• Sea cucumber
• Triterpene glycosides

Holothurian triterpene glycosides possess various kinds of biological activities, including antifungal, cytotoxic, hemolytic, cytostatic, and immunomodulatory effects. In this study, a rapid extraction method of triterpene glycosides from sea cucumbers using a small column of C-18 solid phase was first developed. Furthermore, a novel high-performance liquid chromatography method coupled with evaporative light scattering detection and electrospray ionization mass spectrometry was established for the determination of each triterpene glycosides from different sea cucumbers. Simultaneous separation of all kind of triterpene glycoside were achieved on a C-18 column. A gradient of aqueous acetonitrile was applied, and the method was validated. The liquid chromatography method was applied to the online mass detection to identify the triterpene glycosides in the purified extraction of eight kinds of pulverized sea cucumber from the market of Qingdao, China. The negative mode of [M-H](-)/[M-Na](-) exclusively shown signals corresponding to the triterpene glycosides previously reported and the MS2 product ions of those ions indicate the specific structure of each triterpene glycoside.

• 出版日期2015-4
• 单位中国海洋大学