Assessment of genotoxic and humoral immune system alterations in silica exposed workers from pottery industries in South India

作者：Balamuralikrishnan Balasubramanian^*; Balachandar Vellingiri; Subramaniam Mohana Devi; Alagumuthu Karthick Kumar; Sureshkumar Shanmugam; Arun Meyyazhagan; Arun Sundaramoorthy; Padmavathi Krishnan; Razeena Abdul Hakkim; Gomathi Mohan; Velmurugan Palanivel; Dharwadkar Shahnaz N; Sasikala Keshavarao

来源：Stochastic Environmental Research and Risk Assessment, 2014, 28(7): 1801-1814.

DOI：10.1007/s00477-013-0843-6

• Genetic alterations
• Humoral immunity
• Silica exposure
• Pottery industries

Chemicals, especially silica, have been suspected to cause genetic alterations in pottery industry workers. The present study aims to analyze the frequency of chromosomal aberrations (CA), micronucleus (MN) and DNA damage (comet assay) in the peripheral blood lymphocytes and the immunological alterations workers chronically exposed to silica and in control subjects. In our study, 50 silica exposed workers and 35 control subjects were recruited and silica level was measured by respirable dust and respirable quartz concentrations of badge dosimeter. The serum immunoglobulins (IgM, IgG, and IgA) of all the subjects were measured by using ELISA method. The individuals exposed to silica have a significant increase in the frequency of CA, MN and the total DNA damage (p < 0.05). Immunoglobulin elevation on silica exposed workers was statistically significant (p < 0.05) on comparison with their respective controls. Investigation of the smoking and alcohol habitats coupled with silica exposure in exposed and control subjects represents alcohol consumption and smoking as additional risk factors and must be avoided. Multiple linear regression analysis obtained for CA, MN and comet assay confirm these tests as biomarkers for silica exposed pottery workers. Some confounding factors also showed significant influence on exposed subjects. These results indicate the mutagenic risk in the working environment has a high probability of association with the silica dust exposure in pottery industries. Nevertheless, the present study will create awareness and public concern not only among the silica exposed workers but also to the welfare of their progeny.

• 出版日期2014-10