An antigenic recombinant serine protease from Trichinella spiralis induces protective immunity in BALB/c mice

作者：Li, Xin; Yao, Jian-ping; Pan, Ai-hua; Liu, Wei; Hu, Xu-chu; Wu, Zhong-dao; Zhou, Xing-wang^*

来源：Parasitology Research, 2013, 112(9): 3229-3238.

DOI：10.1007/s00436-013-3500-6

• EXCRETORY-SECRETORY PRODUCTS
• SCHISTOSOMA-MANSONI
• PROTEOLYTIC-ENZYMES
• ANTIBODY-RESPONSE
• ASCARIS-SUUM
• GUINEA-PIG
• IN-VITRO
• TRICHINOSIS
• INFECTION
• PROTEINASES

In this study, we report the cloning and characterization of a cDNA encoding a Trichinella serine protease gene (TspSP-1.3) from GenBank. The recombinant TspSP-1.3 protein (rTspSP-1.3) was expressed in an Escherichia coli expression system and purified with Ni-affinity chromatography. Real-time quantitative PCR analysis revealed that TspSP-1.3 was expressed at significantly higher levels in muscle larvae and adult worms than in newborn larvae. TspSP-1.3 was detected in excretory-secretory proteins of Trichinella spiralis with western blotting. Immunization with the rTspSP-1.3 antigen induced humoral immune responses, which manifested as elevated specific anti-rTspSP-1.3 IgG and IgE antibodies and a mixed Th1/Th2 response. To determine whether purified rTspSP-1.3 had good antigenicity and could be a vaccine candidate for the control of T. spiralis infection, we immunized BALB/c mice with rTspSP-1.3 and subsequently challenged the mice with T. spiralis larvae. The results showed that mice vaccinated with rTspSP-1.3 exhibited an average reduction in the muscle larvae burden of 39 % relative to the control group. These results suggest that TspSP-1.3 could be a novel vaccine candidate for controlling Trichinella infection.

• 出版日期2013-9
• 单位中山大学