Nitric oxide reversibly inhibits Bacillus subtilis oxalate decarboxylase

作者：Moral Mario E G; Tu Chingkuang; Imaram Witcha; Angerhofer Alexander; Silverman David N; Richards Nigel G J^*

来源：Chemical Communications, 2011, 47(11): 3111-3113.

DOI：10.1039/c0cc04946h

Membrane inlet mass spectrometry (MIMS) has been employed to assay the catalytic activity of oxalate decarboxylase (OxDC), allowing us to demonstrate that nitric oxide (NO) reversibly inhibits the enzyme under dioxygen-depleted conditions. X-band EPR measurements do not provide any direct evidence for the interaction of NO with either of the Mn(II) centers in OxDC raising the possibility that there is a separate dioxygen-binding pocket in the enzyme.

• 出版日期2011