Complete Genome Sequencing and Phylogenetic Analysis of a Getah Virus Strain (Genus Alphavirus, Family Togaviridae) Isolated from Culex tritaeniorhynchus Mosquitoes in Nagasaki, Japan in 2012

作者：Kobayashi Daisuke; Isawa Haruhiko^*; Ejiri Hiroko; Sasaki Toshinori; Sunahara Toshihiko; Futami Kyoko; Tsuda Yoshio; Katayama Yukie; Mizutani Tetsuya; Minakawa Noboru; Ohta Nobuo; Sawabe Kyoko

来源：Vector Borne and Zoonotic Diseases, 2016, 16(12): 769-776.

DOI：10.1089/vbz.2016.2017

• Culex tritaeniorhynchus
• Getah virus
• Japan

Getah virus (GETV; genus Alphavirus, family Togaviridae) is a mosquito-borne virus known to cause disease in horses and pigs. In 2014, for the first time in approximate to 30 years, a sudden GETV outbreak occurred among racehorses in Ibaraki, Japan. Two years before this outbreak, we obtained multiple GETV isolates from Culex tritaeniorhynchus mosquitoes collected in Nagasaki, Japan and determined the whole genome sequence of GETV isolate 12IH26. Our phylogenetic analysis of GETV strains revealed that the isolate 12IH26 forms a robust clade with the epidemic strains 14-I-605-C1 and 14-I-605-C2 isolated from horses in the 2014 outbreak in Ibaraki. Furthermore, the complete genomic sequence of the isolate 12IH26 was 99.9% identical to those of the 2014 epidemic strains in Ibaraki. Phylogenetic analysis also showed that the recent Japanese GETV strains, including the isolate 12IH26, are closely related to the Chinese and South Korean strains rather than the previous Japanese strains, suggesting that GETV strains may be transported from overseas into Japan through long-distance migration of the infected mosquitoes or migratory birds.

• 出版日期2016-12