Differential Time Course of Restoration of Experimentally Depleted Coelomocytes and Fluorophores in the Earthworm Eisenia andrei

作者：Santocki Michal; Morgan A John; Plytycz Barbara^*

来源：Folia Biologica-Krakow, 2016, 64(2): 121-130.

DOI：10.3409/fb64_2.121

• earthworms
• coelomocytes
• amoebocytes
• eleocytes
• riboflavin
• MUG fluorophore
• regeneration

Stressed earthworms expel coelomic fluid containing several vital cellular and soluble components, thus their post-stress recovery has adaptive value. The present manuscript describes the recovery rates of coelomocytes (amoebocytes and eleocytes) and two fluorophores (riboflavin and 4-methylumbelliferyl beta-D-glucuronide, MUG) after experimental extrusion by electrostimulation. Analyses were conducted at time points (from 0.5 hour to 7 weeks) by a combination of cell counts, spectrofluorimetric measurements of riboflavin and MUG, and fluorescence microscopy. Coelomic fluid retrieved 30 minutes after extrusion contained <10% of the baseline levels of amoebocytes, eleocytes and riboflavin; the depleted levels of these variables were fully restored after 3, 5, and 7 weeks post-extrusion, respectively. Restored eleocytes were richer in riboflavin than the eleocytes of worms electrostimulated at t(0). MUG was less severely depleted (to 49% of baseline) than riboflavin, and was restored to the initial level within 1 week post-extrusion. This indicates that MUG, unlike riboflavin, resides mainly within non-coelomocyte cellular location(s); moreover, this fluorophore may be a useful molecular marker for distinguishing even immunologically-compromised E. andrei from closely related composting species.

• 出版日期2016-6