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摘要
The requirement for nitric oxide (NO) of lysosomes has motivated the development of a sophisticated fluorescent probe to monitor the distribution of this important biomolecule at the subcellular level in living cells. A near-infrared (NIR) fluorescent Si-rhodamine (SiRB)-NO probe was designed based on the NO-induced ring-opening process of Si-rhodamine. The probe exhibits fast chromogenic and fluorogenic responses, and high sensitivity and selectivity toward trace amounts of NO. Significantly, the spirolactam in Si-rhodamine exhibits very good tolerance to H+, which in turn brings extremely low background fluorescence not only in the physiological environment but also under acidic conditions. The stability of the highly fluorescent product in acidic solution provides persistent fluorescence emission for long-term imaging experiments. To achieve targeted imaging with improved spatial resolution and sensitivity, an efficient lysosome-targeting moiety was conjugated to a SiRB-NO probe, affording a tailored lysosome-targeting NIR fluorescent Lyso-SiRB-NO probe. Inheriting the key advantages of its parent SiRB-NO probe, Lyso-SiRB-NO is a functional probe that is suited for monitoring lysosomal NO with excellent lysosome compatibility. Imaging experiments demonstrated the monitoring of both exogenous and endogenous NO in real time by using the Lyso-SiRB-NO probe.
- 出版日期2016-4-11
- 单位中国人民解放军第二军医大学
