High genetic similarity of ciprofloxacin-resistant Campylobacter jejuni in central Europe

作者：Kovac Jasna; Cadez Neza; Stessl Beatrix; Stingl Kerstin; Gruntar Igor; Ocepek Matjaz; Trkov Marija; Wagner Martin; Mozina Sonja Smole^*

来源：Frontiers in Microbiology, 2015, 6: 1169.

DOI：10.3389/fmicb.2015.01169

• Campylobacter jejuni
• ciprofloxacin resistance
• multilocus sequence typing
• gyrA
• QRDR
• resistance expansion

Campylobacteriosis is the leading zoonosis in the European Union with the majority of cases attributed to Campylobacter jejuni, Although the disease is usually self-limiting, some severe cases need to be treated with antibiotics, primarily macrolides and quinolones. However, the resistance to the latter is reaching alarming levels in most of the EU countries. To shed light on the expansion of antibiotic resistance in central Europe, we have investigated genetic similarity across 178 ciprofloxacin-resistant C. jejuni mostly isolated in Slovenia, Austria and Germany. We performed comparative genetic similarity analyses using allelic types of seven multilocus sequence typing housekeeping genes, and single nucleotide polymorphisms of a quinolone resistance determining region located within the DNA gyrase subunit A gene. This analysis revealed high genetic similarity of isolates from clonal complex ST-21 that carry gyrA allelic type 1 in all three of these central-European countries, suggesting these ciprofloxacin resistant isolates arose from a recent common ancestor and are spread clonally.

• 出版日期2015-10-23