A peptide cross-linked polyacrylamide hydrogel for the detection of human neutrophil elastase

作者：Kamarun Dzaraini; Zheng Xinwei; Milanesi Lilia; Hunter Christopher A; Krause Steffi^*

来源：Electrochimica Acta, 2009, 54(22): 4985-4990.

DOI：10.1016/j.electacta.2009.03.067

• Peptide cross-linked hydrogel
• Polyacrylamide
• Enzymatic degradation
• Human neutrophil elastase
• Quartz crystal microbalance
• Impedance

Polyacrylamide hydrogel films cross-linked with the peptide sequence AAPVAAK were synthesised on piezoelectric quartz crystals. Degradation of the hydrogel films was monitored using a combination of quartz crystal microbalance (QCM) and electrochemical impedance measurements. The films were shown to degrade in the presence of human neutrophil elastase (HNE). The rate of degradation was directly related to the enzyme activity making the system suitable for the detection of HNE in the activity range from 0.72 to 30 U mL(-1). The films were not affected by non-specific adsorption. Film degradation was only accompanied by changes in the QCM signal, while no significant impedance change was observed during degradation.

• 出版日期2009-9-1